New York, US–Mar 13, 2019 With abundant experience in recombinant antibody discovery and manufacturing, Creative Biolabs has been committed to the service about high-quality monoclonal antibodies by the hybridoma technology. Its team of outstanding scientists enables it to generate monoclonal antibodies tailored for its customers’ various needs. In addition, the company is capable of producing human antibodies using transgenic mice.
The monoclonal antibody (mAb) is a highly uniform antibody produced through the cloning of a single B cell, targeting only a specific antigen epitope. In general, the mAb is prepared by use of the hybridomas antibodies technology. Based on the cell fusion technology, this technology allows for the fusion of sensitized B cells with the ability to secrete specific antibodies and myeloma cells capable of reproducing indefinitely, resulting in the production of B cell hybridoma. When such a single hybridoma cell forms a cell population, a specific antibody against an antigen epitope, also known as the mAb, can be prepared.
“Thanks to our advanced hybridoma technology, our company has the ability to provide the one-stop custom production of mAbs. The production goes in four steps. First, antigen preparation. A wide variety of antigens are available at our company. Second, immunization. The prepared antigen may be utilized to immunize animals, thus inducing an immune response. In this step, a comprehensive list of immunization strategies, such as DNA immunization, whole cell immunization, and other conventional immunization strategies, will be ready to fulfill the diverse market demands,” a scientist from Creative Biolabs said.
“Third, hybridoma generation and screening. Multiple approaches, including immunoblotting, immunoprecipitation, ELISA, and IHC methods, can be employed for the efficient hybridoma screening. Fourth, antibody production and purification. Hybridoma cells are inoculated into certain animals to produce ascites. Our hybridoma services boast many advantages, such as high specificity, high affinity, and high accuracy, novel epitope recognition, and comprehensive functional testing. Importantly, there are diverse species for option: mice, rats, hamsters, and guinea pigs.”
On top of the hybridoma technology, Creative Biolabs also excels in the human hybridoma antibodies production using transgenic mice. By inserting human immunoglobulin loci into the murine germlines with inactivated or disrupted endogenous antibody machinery, researchers succeed in obtaining high-affinity full human antibodies, dispensing with the need for the subsequent costly in vitro humanization and affinity maturation. This technology maintains the full humanness of the antibodies, excluding the concerns about immunogenicity.
Thus far, the application of monoclonal antibodies has extended to many medical fields. They play a huge role in the diagnosis, treatment and prevention of a host of major diseases. Meanwhile, the hybridoma services are also improving to satisfy the demands of more niche markets. Besides, the transgenic technology also contributes to the discovery and development of hybridoma antibodies.
About Creative Biolabs
Creative Biolabs is a leading custom service provider, boasting rich experience in antibody production and engineering. Its service portfolio includes mouse and rat monoclonal antibody production using hybridoma technology, human, monkey, rabbit, chicken, dog, llama and camel monoclonal antibody production using various antibody library technologies. In addition, the company also conducts in-depth antibody humanization and affinity maturation.
Media Contact
Company Name: Creative Biolabs
Contact Person: Candy Swift
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Phone: 1-631-619-7922
Address:45-1 Ramsey Road
City: Shirley
State: New York
Country: United States
Website: www.creative-biolabs.com/