Abbkine recently launches a series of Protein Extraction & Quantification Kits, see below listing, more detailed informaton, please click Sampling Kit.

Today I will introduce ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit to you.

Abbkine ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of crude cytoplasmic and nuclear extracts from mammalian cultured cells or tissue. This Kit is based on allowing cells to swell with hypotonic buffer. And then the cells are disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer. Non-denatured, active proteins are purified in less than two hours.

Western blot of specific proteins fraction using Abbkine ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit. Sample: 293T cells lysate. C: cytoplasmic extraction was analyzed using α-tubulin antibody (A01080). N: nuclear extraction was analyzed using α-tubulin antibody (A01080).

Components:

1. Cytoplasmic Solution A (CES A)
2. Cytoplasmic Solution B (CES B)
3. Nuclear Extraction Solution (NES)
4. Protease Inhibitor (100X)
5. DTT (500X)

Features:

1. Versatile: suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations.
2. Fast and convenient: non-denatured, active proteins are purified in less than two hours.
3. Compatible: apply in various downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays.

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