Glass tubes with depyrogenation processing are necessary in the endotoxin test assay to ensure the accuracy and reliability of the test results. Endotoxins are heat-stable molecular components of the outer cell wall of some gram-negative bacteria, and they can cause severe illness and even death in humans if they are present in medical products or devices.

To detect endotoxins, the assay uses reagents that contain Limulus Amebocyte Lysate (LAL) or called Lyophilized amebocyte lysate, an extract from the horseshoe crab’s blood cells that has a clotting mechanism that is activated by endotoxins.

However, glass tubes that have not been depyrogenated can interfere with the LAL test assat by activating its clotting mechanism and producing false positive results. Therefore, glass tubes used in the endotoxin test assay must be depyrogenated to remove any endotoxins that may be present and to prevent activation of the LAL reagent. This ensures that the results of the endotoxin test are accurate and reliable and that patients are not exposed to harmful levels of endotoxins. and ensure the parenteral drugs safety in the pharmaeuticals, protains, cell culture, DNA and so on.

Bioendo Endotoxin-free glass tubes with size 10*75mm, 12*75mm, 13*100mm for the dilutions procedures and reaction procedures.

Endotoxin-free glass tubes are meet the top level standard of endotoxins less than 0.005EU/ml.

Endotoxin-free glass tubes should be used in the gel clot endotoxin test assay to prevent false-positive results.

Endotoxins are bacterial cell wall components that can contaminate laboratory equipment, including glass tubes.

A gel clot endotoxin test assay is used to detect the presence of endotoxins in a sample. In this assay, a clot is formed in the presence of endotoxins. This clot formation is then compared to a control to determine the endotoxin concentration.

Using endotoxin-free glass tubes helps to ensure that the endotoxin detection is accurate. This is because endotoxins can adhere to the surface of glass tubes and interfere with the assay results.

To ensure that the glass tubes used in the gel clot endotoxin test assay are endotoxin-free, they should be washed with a detergent and then thoroughly rinsed with endotoxin-free water. Additionally, they should be sterilized using autoclaving or dry heat sterilization.

In conclusion, using endotoxin-free glass tubes in the gel clot endotoxin test assay is essential to ensure accurate detection of endotoxins. These tubes should be thoroughly cleaned and sterilized to eliminate any potential contamination.

original source: https://www.bioendo.com/news/endotoxin-free-glass-tubes/

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