There are many kinds of loading control antibodies on the market, how to choose the right one for your own experiment based on sample species?
For tissues and cells of mammals, it is generally recommended to choose the proteins like β-actin, Tubulin, GAPDH, Lamin B1, PCNA, Na+/K+-ATPase, etc. Plant actin and Rubisco are often used in plant samples.
1. If you extract total proteins or cytoplasmic proteins from cells, β-actin, GAPDH and Tubulin will be your choice
| Product Name | MW | Catalog No. | Applications |
| Anti-β-Actin Mouse Mab (1C7) | 43 kDa | A01010 | WB, IHC, IF |
| Anti-GAPDH Mouse Mab (2B5) | 37 kDa | A01020 | WB, IHC, IF |
| Anti-β-Tubulin Mouse Mab (3G6) | 55 kDa | A01030 | WB, IHC, IF |
| Anti-α-Tubulin Mab (3G5) | 55 kDa | A01080 | WB, IHC, IF, IP |
| Anti-Cyclophilin B Mab (7B2) | 24 kDa | A01130 | WB, IHC, IF |
| Cofilin Polyclonal Antibody | 19 kDa | ABP51018 | WB, IHC, IF, ELISA |
| Vinculin Polyclonal Antibody | 116 kDa | ABP52701 | WB, IHC, IF, ELISA |
2. For the detection of mitochondrial proteins, you can use the following loading controls
| Product Name | MW | Catalog No. | Applications |
| Anti-COX IV Mouse Mab (14Y2) | 16 kDa | A01060 | WB, IHC, IF |
| HSP60 Rabbit Polyclonal Antibody | 60 kDa | ABP51585 | WB, IHC, IF |
| VDAC1 Rabbit Polyclonal Antibody | 31 kDa | ABP53121 | WB |
3. If your protein of interest is expressed in the nucleus, Histone H3 and PCNA are often selected by researchers
| Product Name | MW | Catalog No. | Applications |
| Anti-PCNA Mouse Mab (1D7) | 29 kDa | A01040 | WB, IHC, IF |
| Anti-Histone H3 Mouse Mab (2D10) | 15 kDa | A01070 | WB, IHC, IF, IP |
| Anti-Lamin B1 Monoclonal Antibody (15T1) | 66 kDa | A01090 | WB, IHC, IF, IP |
| Anti-TBP/TATA Binding Protein Mab (2C6) | 38 kDa | A01120 | WB |
4. For membrane proteins, the commonly used protein is Na+/K+-ATPase; and you can use transferrin for serum samples
| Product Name | MW | Catalog No. | Applications |
| Na+/K+-ATPase α1 Rabbit Pab | 113 kDa | ABP51894 | WB, IHC, IF |
| Transferrin Rabbit Polyclonal Antibody | 77 kDa | ABP52968 | WB, IHC |
5. Plant Actin and Rubisco are the representatives of loading controls for plant samples
| Species | Product name | Catalog No. | Applications |
| Plants | Anti-Plant Actin Mouse Mab (3T3) | A01050 | WB |
| Plant-actin Rabbit Pab | ABP50153 | WB | |
| Anti-Rubisco (Large Chain) Mab (9Y6) | A01110 | WB |
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