PCR (polymerase chain reaction) is one of the fundamental techniques in the field of molecular biology and is widely used for nucleic acid extraction, qPCR and many other applications. The popularity of this technique has led to the development of various PCR sealing membranes, which are used to tightly seal PCR plates or tubes during the process. Suzhou Ace Biomedical Technology Co., Ltd. provides a series of PCR sealing films, including PCR plate optical adhesive sealing film, PCR plate aluminum sealing film, and PCR plate pressure sensitive adhesive sealing film.
Choosing the right sealant for PCR and nucleic acid extraction is critical to successful results. The sealing film prevents contamination and evaporation in the process, which can lead to inaccurate and unreliable results. The following factors should be considered when selecting the appropriate PCR sealant:
compatibility:It is critical to choose a sealant that is compatible with the PCR instrument, tube or plate, and assay chemistry. Compatibility with the temperature and pressure requirements of the experiment is also important.
Material:PCR seals are available in a variety of materials such as optical glue, aluminum, and pressure sensitive adhesive. Each material has unique properties that make it suitable for specific applications. For example, the optical glue sealing film of PCR plate has high light transmittance and penetrability, and is suitable for fluorescence detection. Aluminum PCR plate sealers are ideal for long-term storage, and PCR plate pressure-sensitive adhesive sealers are easy to apply and remove.
thickness:The thickness of the sealing membrane affects the amount of pressure required to seal. Thicker seals may require more force or pressure to seal properly, which may damage the PCR plate or tube. On the other hand, a thinner sealing film can lead to leaks that can lead to contamination in the process.
Easy to use:PCR seals should be easy to use, apply and remove. The sealing film should not stick to the glove or to the PCR plate or tube, making it difficult to remove.
cost:The cost of the sealing film should also be considered as the price will vary depending on the material, thickness and quality of the product. However, the use of low-cost PCR seals may affect the quality of the results.
Suzhou Ace Biomedical Technology Co., Ltd. is a company specializing in the production and sales of PCR sealing film. Their products offer high quality PCR sealing membranes that meet the above standards.
PCR Plate Optical Adhesive Sealing Film: The sealing film has ultra-high optical transparency, can be pierced, and is compatible with various thermal cyclers.
Aluminum sealing film for PCR plate: This sealing film has good air permeability and is suitable for long-term storage.
PCR plate pressure-sensitive adhesive sealing film: This sealing film is easy to use, cost-effective, and compatible with various thermal cyclers.
In summary, choosing the right PCR sealant is critical to obtaining reliable and accurate results. When selecting a sealing film, compatibility, material, thickness, ease of use, and cost must be considered. The PCR plate optical adhesive seal film, PCR plate aluminum seal film, and PCR plate pressure-sensitive adhesive seal film provided by Suzhou Ace Biomedical Technology Co., Ltd. all meet these standards, ensuring the success of PCR and nucleic acid extraction experiments.
article source: https://www.ace-biomedical.com/news/how-to-choose-suitable-sealing-film-for-your-pcr-and-nucleic-acid-extraction/
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