Scientists from CD Genomics have developed a new platform for comprehensive single-cell sequencing—GenSeq. The approach combines its own proprietary techniques and experienced scientists to scale up single-cell sequencing, which explores gene expression in a global pattern and dramatically affect cell biology.
Figure: Single cell sequencing workflow. Source: Wikipedia
What is single-cell sequencing?
By explaining sequencing, we first start with a single cell in genome. We know that the first cell is copied into all the rest – so what’s the difference? For the past decades, scientists have been constantly trying to figure out how genes are turned on and off to perform functions in different cells at different times.
Single-cell sequencing has solved the problem to some extent. It allows scientists to study genes in individual cells, rather than a large piece of tissues. It is a new technology for amplifying and sequencing the DNA/RNA at single cell level.
When studying cells in bulk, the collection seems appear to be uniform but actually have various cell types. Because of this, the research results only reflect an average. By allowing researchers to study just one single cell at a time, single-cell sequencing is redefined as “anatomy”.
“It sifts through cells that appear similar on the surface, highlighting new cell types and vastly improving the ability to find individual genes that drive healthy functions or disease processes in the body”. Said by a Harvard researcher.
Application of single-cell sequencing
- Microbiomes: the technique is the main targets of single cell genomics without cutivation. As the tough part roots in culturing microorganisms in most environments. It has been widely applied on marine, soil, subsurface, organismal, and other types of microbiomes in order to address a wide array of questions related to microbial ecology, evolution, public health and biotechnology potential.
- Cancer sequencing: it is particularly useful for examining the depth of complexity and compound mutations present in amplified therapeutic targets such as receptor tyrosine kinase genes (EGFR, PDGFRA etc.).
- Development, Neurology, Oncology, Autoimmune disease, and Infectious disease.
A recent related study
Single-cell RNA sequencing unveils an IL-10-producing helper subset that sustains humoral immunity during persistent infection: the research focuses on the issue of persistent infection. The authors assessed the heterogeneity of IL-10-expressing CD4 T cells during persistent infection using a single-cell RNA sequencing method. The authors used IL-10 and IL-21 dual reporter mice to demonstrate that IL-10+IL-21+ symbiotic Tfh cells primarily occur during chronic but non-acute LCMV infection. Importantly, deletion of IL-10+IL-21+ symbiotic CD4 T cells or loss of specific Il10 in Tfh cells lead to impaired humoral and viral control, and B cell-specific IL-10 signaling is a germinal center required for the reaction.
Accelerating research
At CD Genomics, the company is dedicated to offering high-quality single-cell RNA sequencing, single-cell DNA sequencing and single-cell DNA methylation sequencing.
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